lightsheet z.1 microscope  (Carl Zeiss)

Journal: Advanced Science

Article Title: Immune and Angiogenic Profiling of Mesenchymal Stem Cell Functions in a Subcutaneous Microenvironment for Allogeneic Islet Transplantation

doi: 10.1002/advs.202411574

Figure Lengend Snippet: Effect of MSCs on islet engraftment and revascularization. EZ Clear processed, wholemount lightsheet fluorescent microscopy imaged NICHE cell reservoir of islet‐loaded devices explanted at days A,B) 7 and C,D) 28; and islets + MSC‐loaded devices explanted at days E,F) 7 and G,H) 28. Islets are stained with insulin‐Alexa Flour 555 (green) and blood vessels are labeled with fluorescently conjugated Lycopersicon esculentum lectin (lectin‐DyLight 649). Scale bars; 1 mm (Top), 400 µm (bottom). I) Islet survival calculated as % of total islet volume loaded in NICHE. Blood vessel volume analysis of J) total cell reservoir and K) intra‐islet volume of no MSC ( n = 3/timepoint) and MSC co‐transplanted ( n = 3 /timepoint) devices. Mean ± SD, two‐way ANOVA with Bonferroni's multiple comparison (* p < 0.05).

Article Snippet: [ ] They were imaged in EZ view solution using a Zeiss Lightsheet Z.1 microscope with a 5× lens at 0.5× zoom.

Techniques: Microscopy, Staining, Labeling, Comparison

Journal: Scientific Reports

Article Title: Phase dependent sympathetic dysinnervation in Takotsubo syndrome revealed with transparent heart

doi: 10.1038/s41598-025-94349-8

Figure Lengend Snippet: Three-dimensional distribution of sympathetic nerve in the heart of Takotsubo syndrome (TTS) mice. ( A ) Bright field image of murine heart after CUBIC procedure. Scale bar, 2 mm. ( B ) Three-dimensional images of tyrosine hydroxylase (TH) (green)-stained and α-smooth muscle actin (α -SMA) (red)-stained heart samples observed using light sheet microscope (Lightsheet Z.1, Carl Zeiss). TH represents sympathetic nerves, and α -SMA is a marker of blood vessels. Scale bar, 2 mm. The right figures show higher magnified views of the boxed region in the left figure. Scale bar, 400 μm. ( C ) Three-dimensional images of the heart obtained from 4-week Sham and 4-week TTS mice. Heart samples were immunostained for TH (green). Sympathetic nerves in the inferior basal area and the inferior apical area were observed by confocal microscope (LSM700, Carl Zeiss). Scale bar, 200 μm. ( D ) Filament analysis of the sympathetic nerves (magenta) in 4-week Sham and 4-week TTS mice using the Imaris software. Scale bar, 200 μm. ( E ) Quantitative analysis of the sympathetic nerves. The ratio of the sympathetic nerve length in the inferior apical area to that in the inferior basal was statistically analyzed between 4-week Sham and 4-week TTS mice (n = 5 each). Statistical significance was determined using the Mann–Whitney U-test. *P < 0.05, vs. 4-week Sham mice. Error bars represent standard error of the mean.

Article Snippet: Scale bar, 2 mm. ( B ) Three-dimensional images of tyrosine hydroxylase (TH) (green)-stained and α-smooth muscle actin (α -SMA) (red)-stained heart samples observed using light sheet microscope (Lightsheet Z.1, Carl Zeiss).

Techniques: Staining, Microscopy, Marker, Software, MANN-WHITNEY